Published on: Sept. 19, 2024

Description: These notes provide a detailed overview of Chromatin Immunoprecipitation followed by sequencing (ChIP-seq), a technique used to study protein-DNA interactions on a genome-wide scale. Key topics include the principles of ChIP-seq, experimental design, sample preparation, antibody selection, and data analysis. The notes also cover common applications, such as identifying transcription factor binding sites, studying histone modifications, and understanding gene regulation mechanisms. This resource serves as a guide for students and researchers to deepen their knowledge of ChIP-seq, from experimental setup to interpreting complex datasets, and applying the technique to various biological questions.